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The various liquid organic extracts (cyclohexanic, 1.42 g; dichloromethanic, 2.53 g; ethyl acetate, 34.85 g; butanolic, 18.97 g and the residual marc, 79.01 g) were then washed with anhydrous sodium sulphate (Fischer Experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals as promulgated by the Senegalese authorities.
Male Wistar rats weighing 150-200 g were procured from a local Institute (Faculté des Sciences et Techniques, Dakar, Senegal).
The extracts were first eluted with 120 ml of methanol/water ().
After the elution of 40 ml of dead volume, fractions of 200 drops are collected (fractions 1 to 11) by means of a fraction collector (Spectra/Chrom CF-1).
In this study, we suggested characterizing the vasodilator effects and the phytochemical characteristics of a plant with food usage also used in traditional treatment of arterial high blood pressure in Senegal.
were evaluated on isolated thoracic aorta of male Wistar rats on organ chambers.
By combining technical preparation (extraction, enrichment, fractionation) and phytochemical characterization (TLC, HPLC) combined with biological characterization methods (organ bath), we strived to identify the phytochemical compounds and estimate their vasorelaxant effects.
calyces was obtained from the Tilène market (Dakar).
The endothelium-dependent relaxations result from NOS activation and those who not dependent to endothelium from activation of smooth muscle potassium channels.
Then elution with 100 ml of methanol/water () for collection of fractions 12 to 23; 100 ml of methanol/water () for collection of fractions 24 to 35; 100 ml of methanol/water () for collection of fractions 36 to 50 and 200 ml of methanol 100% for the collection of the fractions 51 to 67.
Then, the frost was washed with 250 ml of acetone/H2O mixture () to get the fractions 68 to 80.
Indeed, an ethnobotanical investigation led by our laboratory had listed several healing plants with antihypertensive potential among which, , the underlying mechanisms involved as well as the phytochemical compounds responsible for these effects were not fully documented.
So the objective of this study was to contribute to the understanding of such mechanisms and the discovery of bioactive substances responsible for vascular effects of .